Description
Description
MW = 21.4 kDa (dimer). Full length S100β (S100 calcium-binding protein β, S-100 protein beta subunit, S-100 protein beta chain) cloned from human cDNA, expressed in E. coli. The protein consists of the human S100 β (residues 1-92, UniProtKB accession P04271).
Sequence
10 20 30 40 50
MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE
60 70 80 90
QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight below 14.4 kDa.
Supplied as
2 mg/mL solution in Tris 20 mM, pH 7.5, NaCl 150 mM, CaCl2 5 mM. The concentration is estimated by Bradford assay.
Storage
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
References
Cerofolini, L. et al. J. Mol. Recognit. 28 (6), 376-384 (2015).
Markowitz, J. et al. Curr. Top. Med. Chem. 5 (12), 1093-1108 (2005).
Ostendorp, T. et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 61 (7), 673-675 (2005).
Smith, S.P. & Shaw, G.S. Structure 6 (2), 211-222 (1998).
Zimmer, D.B. et al. Brain Res. Bull. 37 (4), 417-429 (1995).
