MMP1 - catalytic domain

Description
MW = 17.6 kDa calculated. Recombinant matrix metalloproteinase-1 (MMP-1, fibroblast collagenase, collagenase-1, interstitial collagenase) cloned from human cDNA, expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-1 (residues 106-261, UniProtKB accession P03956).

Sequence

       110        120        130        140        150
   M-NPRWE QTHLTYRIEN YTPDLPRADV DHAIEKAFQL WSNVTPLTFT 
       160        170        180        190        200
KVSEGQADIM ISFVRGDHRD NSPFDGPGGN LAHAFQPGPG IGGDAHFDED 
       210        220        230        240        250
ERWTNNFREY NLHRVAAHEL GHSLGLSHST DIGALMYPSY TFSGDVQLAQ 
       260       
DDIDGIQAIY G

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 14.4 and 18.4 kDa.

Supplied as
0.2 mg/mL solution in Tris 20 mM, pH 7.2, CaCl₂ 10 mM, ZnCl₂ 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.2 M. The concentration is calculated by the analysis of the absorbance at 280 nm (ε₂₈₀= 28420 M^-1cm^-1 calculated).

Storage
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.

Specific activity
> 100U/μg. Activity described as U = 100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC₂H₅ (Biomol) as substrate.

Usage
Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.

Related Research Fields

• Cancer
  • breast cancer
  • lung cancer
• Dental diseases
  • Pulpitis
• Neurodegenerative diseases
  • Alzheimer’s disease
  • Multiple sclerosis
• Osteoarthritis
• Renal pathologies
  • chronic allograft nephropathy
  • diabetic nephropathy
  • glomerulosclerosis/tubulointerstitial fibrosis
  • polycystic kidney disease
  • renal cell carcinoma
• Wound healing

References

Bertini, I. et al. J. Med. Chem. 48, 7544–7559 (2005).
McCawley, L. J. & Matrisian, L. M. Curr. Opin. Cell Biol. 13, 534–540 (2001).
Stetler-Stevenson, W. G. & Yu, A. E. Semin. Cancer Biol. 11, 143–52 (2001).