Alpha Synuclein

Description

Description
MW = 14.4 kDa calculated. Human, recombinant, Alpha-synuclein (α-Synuclein) cloned from human cDNA, expressed in E. coli. The protein consists of the full length Alpha-synuclein (residues 1-140, UniProtKB accession P37840).

Sequence

        10         20         30         40         50
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH
        60         70         80         90        100 
GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL
       110        120        130        140
GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight of about 14.4 kDa.

Supplied as
0.5 mg/mL solution in sodium phosphate 20 mM pH 6.5, NaCl 200 mM, EDTA 0.5 mM, Complete Protease Inhibitor Cocktail EDTA-free (Roche). The concentration is calculated by the analysis of the absorbance at 280 nm (ε₂₈₀ = 5960 M^-1cm^-1 calculated).

Storage
-80°C. The protein is stable at 4°C for at least 1 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C. Avoid repeated freeze/thaw cycles.

References
Bermel, W. et al. J. Am. Chem. Soc. 128 (12), 3918-3919 (2006).
Norris, E.H. & Giasson, B.I. Antioxid. Redox Signal. 7 (5-6), 672-684 (2005).
Lundvig, D., Lindersson, E. & Jensen, P.H. Mol. Brain Res. 134 (1), 3-17 (2005).
Lee, J.C. et al. Proc. Natl. Acad. Sci. 101 (47), 16466-16471 (2004).
Fernández, C.O. et al. EMBO J. 23 (10), 2039-2046 (2004).
Cuervo, A.M. Science 305 (5688), 1292-1295 (2004).
Xia, Y. et al. J. Alzheimer’s Dis. 3 (5), 485-494 (2001).