S100A16

Description
MW = 23.6 kDa (dimer) calculated. Full length S100A16 (S100 calcium-binding protein A16, Protein S100F, Aging-associated gene 13 protein, S10AG) cloned from human cDNA, expressed in E. coli. The protein consists of the human S100A16 (residues 1-103, UniProtKB accession Q96FQ6).

 

Sequence

        10         20         30         40         50
MSDCYTELEK AVIVLVENFY KYVSKYSLVK NKISKSSFRE MLQKELNHML 
        60         70         80         90        100
SDTGNRKAAD KLIQNLDANH DGRISFDEYW TLIGGITGPI AKLIHEQEQQ 

SSS

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight below 14.4 kDa.

Supplied as
1 mg/mL solution in MES 20 mM pH 5.5, KCl 200 mM, DTT 1 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε₂₈₀ = 12950 M^-1cm^-1 calculated).

Storage
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.

References
Sturchler, E. et al. J. Biol. Chem. 281 (50), 38905-38917 (2006).
Marenholz, I. & Heizmann, C.W. Biochem. Biophys. Res. Commun. 313 (2), 237-244 (2004).