S100A4

Description
MW = 23.4 kDa (dimer) calculated. Full length S100A4 (S100 calcium-binding protein A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1) cloned from human cDNA, expressed in E. coli. The protein consists of the human S100A4 (residues 1-101, UniProtKB accession P26447).

 

Sequence

        10         20         30         40         50
MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT 
        60         70         80         90        100
DEAAFQKLMS NLDSNRDNEV DFQEYCVFLS CIAMMCNEFF EGFPDKQPRK 

K

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight below 14.4 kDa.

Supplied as
1 mg/mL solution in Tris 20 mM, pH 7.5, NaCl 150 mM, CaCl₂ 5 mM, DTT 2 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε₂₈₀ = 2980 M^-1cm^-1 calculated).

Storage
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.

References
Engelkamp D., et al. Biochemistry 31:10258-10264(1992)
Vallely K.M., et al. Biochemistry 41:12670-12680 (2002)
Boye K, et al. Am J Pathol. Feb;176(2):528-35(2010)