Additional information
| Labeling | 13C15N |
|---|---|
| NMR Standard | 3 mm NMR tube, 5 mm NMR tube |
| Shipping in Dry Ice | no |
SH3 > SH3 - 13C15N in NMR tube
From Chicken, recombinant
Rresidues 965-1025, UniProtKB accession number P07751
MW = 7.2 kDa
CAT # G99SH301cn
| Catalog n. | NMR Standard | Labeling | Price |
|---|---|---|---|
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Description
Description
MW = 7.2kDa. Recombinant α-Spectrin SH3 domain (Spectrin, non-erythroid alpha chain, Fodrin alpha chain), cloned from cDNA from chicken and expressed in E. coli. The protein consists of the soluble SH3 domain of α-Spectrin from Gallus gallus (residues 965-1025). UniProtKB accession P07751.
MW = 7.2kDa. Recombinant α-Spectrin SH3 domain (Spectrin, non-erythroid alpha chain, Fodrin alpha chain), cloned from cDNA from chicken and expressed in E. coli. The protein consists of the soluble SH3 domain of α-Spectrin from Gallus gallus (residues 965-1025). UniProtKB accession P07751.
Sequence
970 980 990 1000
M-DETGKE LVLALYDYQE KSPREVTMKK GDILTLLNST
1010 1020
NKDWWKVEVN DRQGFVPAAY VKKLD
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight below 14.4 kDa.
Supplied as
1 mg/mL solution in sodium citrate 10 mM pH 3.5, NaN3 0.02% , D2O 10%. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 15470 M-1cm-1 calculated).
(unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)
Storage
-20°C. The protein is stable at 4°C for months. Avoid repeated freeze/thaw cycles.
NMR USE: after the experiments return the sample at 4°C.
References
Blanco, F.J., Ortiz, Á.R. & Serrano, L. J. Biomol. NMR 9 (4), 347-357 (1997).